A scanning laser is used to excite the fluorophore as in a regular confocal system, but a second “doughnut” shaped laser is superimposed with this laser and causes a depletion of fluorescence inside that area. Super Resolution is achieved using Stimulated Emission Depletion (STED) microscopy. The use a confocal pinhole prevents out of focus light from reaching the detector and provides a complete 3D volume of the sample that is free from blur with a high level of resolution. Zeiss LSM 880 upright AiryScan confocalĬonfocal microscopy works by rastering a single diffraction limited laser spot across the sample so that excitation and detection is achieved “point by point” to build up the complete image.Zeiss LSM 880 inverted AiryScan confocal with multi-photon.Zeiss LSM 880 inverted AiryScan confocal with FCS.
Leica SP8 WLL 3x gSTED super resolution confocal with FLIM and FCS.Leica SP8 upright multi-photon capable of in vivo imaging.Leica SP8 WLL upright confocal (dipping lenses, Clarity lens).Leica SP8 WLL inverted confocal with High Content Screening (HCS) capability.
